Diagnostic Reverse-Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories
Identifieur interne : 000286 ( France/Analysis ); précédent : 000285; suivant : 000287Diagnostic Reverse-Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories
Auteurs : Marcus Panning [Allemagne] ; Thomas Laue [Allemagne] ; Stephan Ölschlager [Allemagne] ; Markus Eickmann [Allemagne] ; Stephan Becker [Allemagne] ; Sabine Raith [Allemagne] ; Marie-Claude Georges Courbot [France] ; Mikael Nilsson [Suède] ; Robin Gopal [Royaume-Uni] ; Ake Lundkvist [Suède] ; Antonino Di Caro [Italie] ; David Brown [Royaume-Uni] ; Hermann Meyer [Allemagne] ; Graham Lloyd [Royaume-Uni] ; Beate M. Kümmerer [Allemagne] ; Stephan Günther [Allemagne] ; Christian Drosten [Allemagne]Source :
- The Journal of Infectious Diseases [ 0022-1899 ] ; 2007.
Abstract
A network of European biosafety level 4 laboratories has designed the first industry-standard molecular assay for all filoviruses species, based on the strain collections of all participants. It uses 5 optimized L gene primers and 3 probes, as well as an internal control with a separate detection probe. Detection limits (probit analysis, 95% detection chance) were as follows: Zaire ebolavirus, 487 copies/mL of plasma; Sudan ebolavirus Maleo, 586 copies/mL; Sudan ebolavirus Gulu, 1128 copies/mL; Cote d'Ivoire ebolavirus, 537 copies/mL; Reston ebolavirus, 4546 copies/mL; Lake Victoria marburgvirus Musoke, 860 copies/mL; and Lake Victoria marburgvirus Ravn, 1551 copies/mL. The assay facilitates reliable detection or exclusion screening of filovirus infections.
Url:
DOI: 10.1086/520600
Affiliations:
- Allemagne, France, Italie, Royaume-Uni, Suède
- Angleterre, Auvergne-Rhône-Alpes, Bavière, District de Giessen, District de Haute-Bavière, Grand Londres, Hambourg, Hesse (Land), Latium, Rhône-Alpes
- Hambourg, Londres, Lyon, Marbourg, Munich, Rome
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000C10
- to stream Istex, to step Curation: 000C10
- to stream Istex, to step Checkpoint: 001175
- to stream Main, to step Merge: 003986
- to stream Main, to step Curation: 003832
- to stream Main, to step Exploration: 003832
- to stream France, to step Extraction: 000286
Links to Exploration step
ISTEX:132FFE9AD7A1A2EEDB40F77F78284659D4FF368ALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Diagnostic Reverse-Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories</title><author><name sortKey="Panning, Marcus" sort="Panning, Marcus" uniqKey="Panning M" first="Marcus" last="Panning">Marcus Panning</name></author><author><name sortKey="Laue, Thomas" sort="Laue, Thomas" uniqKey="Laue T" first="Thomas" last="Laue">Thomas Laue</name></author><author><name sortKey="Olschlager, Stephan" sort="Olschlager, Stephan" uniqKey="Olschlager S" first="Stephan" last="Ölschlager">Stephan Ölschlager</name></author><author><name sortKey="Eickmann, Markus" sort="Eickmann, Markus" uniqKey="Eickmann M" first="Markus" last="Eickmann">Markus Eickmann</name></author><author><name sortKey="Becker, Stephan" sort="Becker, Stephan" uniqKey="Becker S" first="Stephan" last="Becker">Stephan Becker</name></author><author><name sortKey="Raith, Sabine" sort="Raith, Sabine" uniqKey="Raith S" first="Sabine" last="Raith">Sabine Raith</name></author><author><name sortKey="Courbot, Marie Claude Georges" sort="Courbot, Marie Claude Georges" uniqKey="Courbot M" first="Marie-Claude Georges" last="Courbot">Marie-Claude Georges Courbot</name></author><author><name sortKey="Nilsson, Mikael" sort="Nilsson, Mikael" uniqKey="Nilsson M" first="Mikael" last="Nilsson">Mikael Nilsson</name></author><author><name sortKey="Gopal, Robin" sort="Gopal, Robin" uniqKey="Gopal R" first="Robin" last="Gopal">Robin Gopal</name></author><author><name sortKey="Lundkvist, Ake" sort="Lundkvist, Ake" uniqKey="Lundkvist A" first="Ake" last="Lundkvist">Ake Lundkvist</name></author><author><name sortKey="Di Caro, Antonino" sort="Di Caro, Antonino" uniqKey="Di Caro A" first="Antonino" last="Di Caro">Antonino Di Caro</name></author><author><name sortKey="Brown, David" sort="Brown, David" uniqKey="Brown D" first="David" last="Brown">David Brown</name></author><author><name sortKey="Meyer, Hermann" sort="Meyer, Hermann" uniqKey="Meyer H" first="Hermann" last="Meyer">Hermann Meyer</name></author><author><name sortKey="Lloyd, Graham" sort="Lloyd, Graham" uniqKey="Lloyd G" first="Graham" last="Lloyd">Graham Lloyd</name></author><author><name sortKey="Kummerer, Beate M" sort="Kummerer, Beate M" uniqKey="Kummerer B" first="Beate M." last="Kümmerer">Beate M. Kümmerer</name></author><author><name sortKey="Gunther, Stephan" sort="Gunther, Stephan" uniqKey="Gunther S" first="Stephan" last="Günther">Stephan Günther</name></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:132FFE9AD7A1A2EEDB40F77F78284659D4FF368A</idno><date when="2007" year="2007">2007</date><idno type="doi">10.1086/520600</idno><idno type="url">https://api.istex.fr/ark:/67375/HXZ-GF4DFKKG-B/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000C10</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000C10</idno><idno type="wicri:Area/Istex/Curation">000C10</idno><idno type="wicri:Area/Istex/Checkpoint">001175</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001175</idno><idno type="wicri:doubleKey">0022-1899:2007:Panning M:diagnostic:reverse:transcription</idno><idno type="wicri:Area/Main/Merge">003986</idno><idno type="wicri:Area/Main/Curation">003832</idno><idno type="wicri:Area/Main/Exploration">003832</idno><idno type="wicri:Area/France/Extraction">000286</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Diagnostic Reverse-Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories</title><author><name sortKey="Panning, Marcus" sort="Panning, Marcus" uniqKey="Panning M" first="Marcus" last="Panning">Marcus Panning</name><affiliation wicri:level="3"><country>Allemagne</country><placeName><settlement type="city">Hambourg</settlement><region type="land" nuts="2">Hambourg</region></placeName><wicri:orgArea>Bernhard-Nocht Institute for Tropical Medicine</wicri:orgArea></affiliation></author><author><name sortKey="Laue, Thomas" sort="Laue, Thomas" uniqKey="Laue T" first="Thomas" last="Laue">Thomas Laue</name><affiliation wicri:level="3"><country>Allemagne</country><placeName><settlement type="city">Hambourg</settlement><region type="land" nuts="2">Hambourg</region></placeName><wicri:orgArea>Qiagen</wicri:orgArea></affiliation></author><author><name sortKey="Olschlager, Stephan" sort="Olschlager, Stephan" uniqKey="Olschlager S" first="Stephan" last="Ölschlager">Stephan Ölschlager</name><affiliation wicri:level="3"><country>Allemagne</country><placeName><settlement type="city">Hambourg</settlement><region type="land" nuts="2">Hambourg</region></placeName><wicri:orgArea>Bernhard-Nocht Institute for Tropical Medicine</wicri:orgArea></affiliation></author><author><name sortKey="Eickmann, Markus" sort="Eickmann, Markus" uniqKey="Eickmann M" first="Markus" last="Eickmann">Markus Eickmann</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>University of Marburg, Marburg</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Giessen</region><settlement type="city">Marbourg</settlement></placeName></affiliation></author><author><name sortKey="Becker, Stephan" sort="Becker, Stephan" uniqKey="Becker S" first="Stephan" last="Becker">Stephan Becker</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>University of Marburg, Marburg</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Giessen</region><settlement type="city">Marbourg</settlement></placeName></affiliation></author><author><name sortKey="Raith, Sabine" sort="Raith, Sabine" uniqKey="Raith S" first="Sabine" last="Raith">Sabine Raith</name><affiliation wicri:level="3"><country>Allemagne</country><placeName><settlement type="city">Hambourg</settlement><region type="land" nuts="2">Hambourg</region></placeName><wicri:orgArea>Qiagen</wicri:orgArea></affiliation></author><author><name sortKey="Courbot, Marie Claude Georges" sort="Courbot, Marie Claude Georges" uniqKey="Courbot M" first="Marie-Claude Georges" last="Courbot">Marie-Claude Georges Courbot</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Unité de Biologie des Infections Virales Emergentes, Institut Pasteur, Lyon</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Nilsson, Mikael" sort="Nilsson, Mikael" uniqKey="Nilsson M" first="Mikael" last="Nilsson">Mikael Nilsson</name><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Swedish Centre for Infectious Disease Control, Solna</wicri:regionArea><wicri:noRegion>Solna</wicri:noRegion></affiliation></author><author><name sortKey="Gopal, Robin" sort="Gopal, Robin" uniqKey="Gopal R" first="Robin" last="Gopal">Robin Gopal</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Health Protection Agency, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Lundkvist, Ake" sort="Lundkvist, Ake" uniqKey="Lundkvist A" first="Ake" last="Lundkvist">Ake Lundkvist</name><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Swedish Centre for Infectious Disease Control, Solna</wicri:regionArea><wicri:noRegion>Solna</wicri:noRegion></affiliation></author><author><name sortKey="Di Caro, Antonino" sort="Di Caro, Antonino" uniqKey="Di Caro A" first="Antonino" last="Di Caro">Antonino Di Caro</name><affiliation wicri:level="3"><country xml:lang="fr">Italie</country><wicri:regionArea>National Institute for Infectious Diseases “L. Spallanzani”, Rome</wicri:regionArea><placeName><settlement type="city">Rome</settlement><region nuts="2">Latium</region></placeName></affiliation></author><author><name sortKey="Brown, David" sort="Brown, David" uniqKey="Brown D" first="David" last="Brown">David Brown</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Health Protection Agency, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Meyer, Hermann" sort="Meyer, Hermann" uniqKey="Meyer H" first="Hermann" last="Meyer">Hermann Meyer</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Bundeswehr Institute of Microbiology, Munich</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Haute-Bavière</region><settlement type="city">Munich</settlement></placeName></affiliation></author><author><name sortKey="Lloyd, Graham" sort="Lloyd, Graham" uniqKey="Lloyd G" first="Graham" last="Lloyd">Graham Lloyd</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Health Protection Agency Porton Down, Salisbury</wicri:regionArea><wicri:noRegion>Salisbury</wicri:noRegion></affiliation></author><author><name sortKey="Kummerer, Beate M" sort="Kummerer, Beate M" uniqKey="Kummerer B" first="Beate M." last="Kümmerer">Beate M. Kümmerer</name><affiliation wicri:level="3"><country>Allemagne</country><placeName><settlement type="city">Hambourg</settlement><region type="land" nuts="2">Hambourg</region></placeName><wicri:orgArea>Bernhard-Nocht Institute for Tropical Medicine</wicri:orgArea></affiliation></author><author><name sortKey="Gunther, Stephan" sort="Gunther, Stephan" uniqKey="Gunther S" first="Stephan" last="Günther">Stephan Günther</name><affiliation wicri:level="3"><country>Allemagne</country><placeName><settlement type="city">Hambourg</settlement><region type="land" nuts="2">Hambourg</region></placeName><wicri:orgArea>Bernhard-Nocht Institute for Tropical Medicine</wicri:orgArea></affiliation></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><affiliation wicri:level="3"><country>Allemagne</country><placeName><settlement type="city">Hambourg</settlement><region type="land" nuts="2">Hambourg</region></placeName><wicri:orgArea>Bernhard-Nocht Institute for Tropical Medicine</wicri:orgArea></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Allemagne</country></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Allemagne</country><wicri:regionArea>Reprints or correspondence: Dr. Christian Drosten, Bernhard Nocht Institute for Tropical Medicine, Clinical Virology, Bernhard-Nocht Str. 74, 20359 Hamburg</wicri:regionArea><placeName><settlement type="city">Hambourg</settlement><region type="land" nuts="2">Hambourg</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">The Journal of Infectious Diseases</title><title level="j" type="abbrev">The Journal of Infectious Diseases</title><idno type="ISSN">0022-1899</idno><idno type="eISSN">1537-6613</idno><imprint><publisher>The University of Chicago Press</publisher><date type="published">2007</date><biblScope unit="vol">196</biblScope><biblScope unit="issue">Supplemet_2</biblScope><biblScope unit="page" from="S199">S199</biblScope><biblScope unit="page" to="S204">S204</biblScope></imprint><idno type="ISSN">0022-1899</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1899</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">A network of European biosafety level 4 laboratories has designed the first industry-standard molecular assay for all filoviruses species, based on the strain collections of all participants. It uses 5 optimized L gene primers and 3 probes, as well as an internal control with a separate detection probe. Detection limits (probit analysis, 95% detection chance) were as follows: Zaire ebolavirus, 487 copies/mL of plasma; Sudan ebolavirus Maleo, 586 copies/mL; Sudan ebolavirus Gulu, 1128 copies/mL; Cote d'Ivoire ebolavirus, 537 copies/mL; Reston ebolavirus, 4546 copies/mL; Lake Victoria marburgvirus Musoke, 860 copies/mL; and Lake Victoria marburgvirus Ravn, 1551 copies/mL. The assay facilitates reliable detection or exclusion screening of filovirus infections.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>France</li><li>Italie</li><li>Royaume-Uni</li><li>Suède</li></country><region><li>Angleterre</li><li>Auvergne-Rhône-Alpes</li><li>Bavière</li><li>District de Giessen</li><li>District de Haute-Bavière</li><li>Grand Londres</li><li>Hambourg</li><li>Hesse (Land)</li><li>Latium</li><li>Rhône-Alpes</li></region><settlement><li>Hambourg</li><li>Londres</li><li>Lyon</li><li>Marbourg</li><li>Munich</li><li>Rome</li></settlement></list><tree><country name="Allemagne"><region name="Hambourg"><name sortKey="Panning, Marcus" sort="Panning, Marcus" uniqKey="Panning M" first="Marcus" last="Panning">Marcus Panning</name></region><name sortKey="Becker, Stephan" sort="Becker, Stephan" uniqKey="Becker S" first="Stephan" last="Becker">Stephan Becker</name><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><name sortKey="Eickmann, Markus" sort="Eickmann, Markus" uniqKey="Eickmann M" first="Markus" last="Eickmann">Markus Eickmann</name><name sortKey="Gunther, Stephan" sort="Gunther, Stephan" uniqKey="Gunther S" first="Stephan" last="Günther">Stephan Günther</name><name sortKey="Kummerer, Beate M" sort="Kummerer, Beate M" uniqKey="Kummerer B" first="Beate M." last="Kümmerer">Beate M. Kümmerer</name><name sortKey="Laue, Thomas" sort="Laue, Thomas" uniqKey="Laue T" first="Thomas" last="Laue">Thomas Laue</name><name sortKey="Meyer, Hermann" sort="Meyer, Hermann" uniqKey="Meyer H" first="Hermann" last="Meyer">Hermann Meyer</name><name sortKey="Olschlager, Stephan" sort="Olschlager, Stephan" uniqKey="Olschlager S" first="Stephan" last="Ölschlager">Stephan Ölschlager</name><name sortKey="Raith, Sabine" sort="Raith, Sabine" uniqKey="Raith S" first="Sabine" last="Raith">Sabine Raith</name></country><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Courbot, Marie Claude Georges" sort="Courbot, Marie Claude Georges" uniqKey="Courbot M" first="Marie-Claude Georges" last="Courbot">Marie-Claude Georges Courbot</name></region></country><country name="Suède"><noRegion><name sortKey="Nilsson, Mikael" sort="Nilsson, Mikael" uniqKey="Nilsson M" first="Mikael" last="Nilsson">Mikael Nilsson</name></noRegion><name sortKey="Lundkvist, Ake" sort="Lundkvist, Ake" uniqKey="Lundkvist A" first="Ake" last="Lundkvist">Ake Lundkvist</name></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Gopal, Robin" sort="Gopal, Robin" uniqKey="Gopal R" first="Robin" last="Gopal">Robin Gopal</name></region><name sortKey="Brown, David" sort="Brown, David" uniqKey="Brown D" first="David" last="Brown">David Brown</name><name sortKey="Lloyd, Graham" sort="Lloyd, Graham" uniqKey="Lloyd G" first="Graham" last="Lloyd">Graham Lloyd</name></country><country name="Italie"><region name="Latium"><name sortKey="Di Caro, Antonino" sort="Di Caro, Antonino" uniqKey="Di Caro A" first="Antonino" last="Di Caro">Antonino Di Caro</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000286 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000286 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= France
|étape= Analysis
|type= RBID
|clé= ISTEX:132FFE9AD7A1A2EEDB40F77F78284659D4FF368A
|texte= Diagnostic Reverse-Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories
}}
| This area was generated with Dilib version V0.6.33. |  |